Question

Sometimes mutagenesis is carried out with the help of primers. Choose the correct statement with respect to it.

(a) Double stranded circular molecule is used as a template

(b) Mutation is introduced into one of flanking primers

(c) Single stranded circular molecule is used as a template

(d) After amplification, mutation is introduced into one strand

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Answer 1

Right choice is (a) Double stranded circular molecule is used as a template

To elaborate: Double stranded circular molecules are used as a template. Mutation is introduced into both of the primers and then amplification is carried out. After amplification is carried out, mutation can be introduced either in one strand or both the strands.