Question

PCR using the mutagenic primer and one of the flanking primers is used to carry out amplification and generates a product corresponding to the part of the gene. It is called as megaprimer.

(a) True

(b) False

The question was asked during an internship interview.

This key question is from Mutagenesis using PCR and Recovery of Mutated Sequences in chapter Modifications and Mutagenesis of Genetic Engineering

Answer 1

Correct option is (a) True

To elaborate: Megaprimer is produced when amplification is carried out by using mutagenic primer and one of the flanking primers. It corresponds to the part of the gene.