Login

Remember

Register

CarrierAdda QnA

Q&A
Hot!
Unanswered
Tags
Users
Ask a Question

Home
Genetic Engineering
Once second strand synthesis is carried out, it...

Once second strand synthesis is carried out, it is introduced into the host. Host is having which mutation?

← Prev Question Next Question →

+1 vote

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

Once second strand synthesis is carried out, it is introduced into the host. Host is having which mutation?

(a) mutS mutation

(b) mutD mutation

(c) mutE mutation

(d) mutG mutation

This question was addressed to me during an online exam.

This intriguing question originated from Mutagenesis using PCR and Recovery of Mutated Sequences in chapter Modifications and Mutagenesis of Genetic Engineering

genetic engineering
modifications and mutagenesis
mutagenesis using pcr and recovery of mutated sequences

Facebook Twitter LinkedIn

Please log in or register to answer this question.

1 Answer

+1 vote

answered Feb 9, 2022 by Noah (693k points)
selected Sep 18, 2022 by Charlotte

Best answer

Right option is (a) mutS mutation

To explain: The host is having a mutS mutation. And because of this mutation, no mismatch repair takes place.

Please log in or register to add a comment.

Related questions

+1 vote

1 answer

Sometimes mutagenesis is carried out with the help of primers. Choose the correct statement with respect to it.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
mutagenesis using pcr and recovery of mutated sequences

+1 vote

1 answer

Once the double stranded molecule with the mutation is introduced into E. coli for replication, how many types of molecules are produced?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
mutagenesis without pcr

+1 vote

1 answer

Replication by first strand leads to the formation of mutated molecules and functional antibiotic resistant gene.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
mutagenesis using pcr and recovery of mutated sequences

+1 vote

1 answer

PCR using the mutagenic primer and one of the flanking primers is used to carry out amplification and generates a product corresponding to the part of the gene. It is called as megaprimer.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
mutagenesis using pcr and recovery of mutated sequences

+1 vote

1 answer

Which of the following statement is incorrect for the synthesis of the second strand?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
mutagenesis without pcr

+1 vote

1 answer

For the selection of the molecules having mutated sequence, which of the statement is true?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
mutagenesis using pcr and recovery of mutated sequences

+1 vote

1 answer

Choose the correct statement. (a)There are two flanking primers and they are having mutations

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
mutagenesis using pcr and recovery of mutated sequences

+1 vote

1 answer

If PCR is used to introduce random mutations rather than specific mutations, it is called as ___________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
mutagenesis using pcr and recovery of mutated sequences

+1 vote

1 answer

A megaprimer method is a ____ stage approach and uses _____ oligonucleotide primers.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
mutagenesis using pcr and recovery of mutated sequences

+1 vote

1 answer

What is the function of ung gene?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
mutagenesis using pcr and recovery of mutated sequences

+1 vote

1 answer

In a phosphothiorate nucleotide, oxygen atom is replaced by with atom?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
mutagenesis using pcr and recovery of mutated sequences

+1 vote

1 answer

DpnI cuts ________ strands.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
mutagenesis using pcr and recovery of mutated sequences

+1 vote

1 answer

Choose the incorrect statement for the methodology of selection of molecules with mutant sequences.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
mutagenesis using pcr and recovery of mutated sequences

+1 vote

1 answer

Introduction into host organism can also be done by using a DNA construct, which when transcribed, generates an RNA which is _________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
post-transcriptional gene silencing

+1 vote

1 answer

Screening is often carried out for a sequence that interacts with a protein for which already a clone is present. It is carried out in which host?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
screening libraries
reporter genes

+1 vote

1 answer

If there are multiple restriction sites for an enzyme in a given molecule, digestion is carried out in many steps. The initial digestion should be?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
removal and introduction of restriction sites and generation of insertions and deletions

+1 vote

1 answer

Once the bacteriophage enters the bacterial cells, some changes are carried out in it. Which of the following is not included?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
other vector systems for e
bac vector
m13 and its derivatives

+1 vote

1 answer

Cre can be introduced by crossing it with a strain containing the gene or by infection with virus containing it.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
cre lox excision

+1 vote

1 answer

Choose the incorrect statement for second DNA strand synthesis.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
libraries construction
cdna libraries
polyadenylated rna and cdna synthesis

+1 vote

1 answer

An oligonucleotide is synthesized which contains the mutation and the rest is _______ to the template DNA.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
mutagenesis without pcr

+1 vote

1 answer

It is often useful to inactivate endogenous genes in an organism. It might be helpful in finding out _________ role of the wild type gene.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
choosing the right mutations
database of mutant lines and gene disruption

+1 vote

1 answer

If hybridization is carried out with a clone having a sequence similar to that of the vector from which library has been constructed, then it is of no use. Choose the correct statement in respect to it.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
screening libraries
database screening and screening by nucleic acid hybridisation

+1 vote

1 answer

Vector and insert are mixed, ligated and packaged and introduced into the host by ___________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
libraries construction
genomic libraries

+1 vote

1 answer

Restriction site can also be introduced by oligonucleotide directed mutagenesis of a region that is _______ and ______ to the original restriction site.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
removal and introduction of restriction sites and generation of insertions and deletions

+1 vote

1 answer

Additional restriction sites can be introduced near the existing restriction site by mutagenesis.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
removal and introduction of restriction sites and generation of insertions and deletions

Facebook Twitter LinkedIn

We welcome you to Carrieradda QnA with open heart. Our small community of enthusiastic learners are very helpful and supportive. Here on this platform you can ask questions and receive answers from other members of the community. We also monitor posted questions and answers periodically to maintain the quality and integrity of the platform. Hope you will join our beautiful community

Categories

All categories
Environmental Biotechnology (884)
Environmental Engineering (1.1k)
Enzyme Technology (752)
Farm Machinery (534)
Fermentation Technology (994)
Finite Element Method (480)
Fluid Mechanics (948)
Fluidization Engineering (267)
Food Engineering (984)
Food Packaging Technology (502)
Food Processing Unit Operations (663)
Foundry and Forging (381)
Genetic Engineering (710)
Geotechnical Engineering I (1.2k)
Geotechnical Engineering II (735)
Hazardous Waste Management (880)
Software Architecture (921)
Mass Transfer (499)
Machine Dynamics (772)
Machine Kinematics (912)
Irrigation Engineering (1.2k)
Linear Integrated Circuits (404)
Machine Design (0)
Machine Drawing (0)

Send feedback
About Us
Copyright
Privacy Policy
Terms of Use
Contact Us

Powered by TalkJarvis

...