Question

Choose the correct statement.

(a) There are two flanking primers and they are having mutations

(b) There is one primer which anneals the target sequence and is having mutation

(c) Either of the flanking primers or the primer is annealing to the target sequence is having mutation

(d) One of the flanking primer and the primer annealing to the target sequence is having mutation

I had been asked this question in an online interview.

This interesting question is from Mutagenesis using PCR and Recovery of Mutated Sequences in division Modifications and Mutagenesis of Genetic Engineering

Answer 1

Right option is (b) There is one primer which anneals the target sequence and is having mutation

Easiest explanation: In the megaprimer approach three primers are used. There are two primers which flank the target sequence and a third primer anneal to the target sequence. The mutation is there in the third primer.