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An oligonucleotide is synthesized which contains the mutation and the rest is _______ to the template DNA.

(a) complementary

(b) non-complementary

(c) can either be complementary or non-complementary

(d) not related

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This interesting question is from Mutagenesis without PCR topic in section Modifications and Mutagenesis of Genetic Engineering

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Best answer
Right option is (a) complementary

Best explanation: The oligonucleotide is synthesized which contains the mutation and the rest of the sequence is complementary to the template DNA. And the oligonucleotide is allowed to anneal to the template DNA.

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