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For mutagenesis without PCR, which of the following can be used as a template?

(a) Single stranded DNA

(b) Double stranded DNA

(c) Circular DNA

(d) Both single and double stranded DNA

I got this question in an online quiz.

The above asked question is from Mutagenesis without PCR in section Modifications and Mutagenesis of Genetic Engineering

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Right answer is (d) Both single and double stranded DNA

For explanation I would say: If mutagenesis is carried without PCR, both single and double stranded DNA can be used as a template. The single stranded DNA is obtained from filamentous phages and double stranded DNA is obtained from conventional vectors.

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