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If mixed oligonucleotides are used, it is regarded as ______________

(a) mixed mutagenesis

(b) multiple mutagenesis

(c) cassette mutagenesis

(d) polymutagenesis

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I'd like to ask this question from Mutagenesis without PCR topic in division Modifications and Mutagenesis of Genetic Engineering

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Correct option is (c) cassette mutagenesis

Easiest explanation: Mixed oligomucleotides is the collection of those oligonucleotides which are having different nucleotides at the same position. This type of mutagenesis is called as cassette mutagenesis. It is a fast method for inducing multiple mutations.

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