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The disrupted gene is excised from the vector and is inserted into the target organism. The excised gene should be in which form?

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The disrupted gene is excised from the vector and is inserted into the target organism. The excised gene should be in which form?

(a) Circular

(b) Supercoiled

(c) Either supercoiled or circular

(d) Linear

I had been asked this question during an internship interview.

This is a very interesting question from Choosing the Right Mutations, Database of Mutant Lines and Gene Disruption in portion Modifications and Mutagenesis of Genetic Engineering

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Correct answer is (d) Linear

The best I can explain: The disrupted gene is excised from the vector and is inserted into the target organism. The excised gene should be linear in form.

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