Question

In the case of deletion of a restriction site, it should be cleaved with the same restriction enzyme.

(a) True

(b) False

I got this question by my college director while I was bunking the class.

My doubt stems from Removal and Introduction of Restriction Sites and Generation of Insertions and Deletions topic in division Modifications and Mutagenesis of Genetic Engineering

Answer 1

Right answer is (a) True

Best explanation: For the deletion of a restriction site, it should be cleaved with the same restriction enzyme. As it is cleaved, the restriction site is destroyed and further the staggered ends created are either filled or degraded. After this religation is carried out but the restriction site is destroyed.