Question

At times, the gene which is cloned is not well known for the protein encoded by it. To access the function, the endogenous gene for the mutant strain is inactivated. This technique is called as ___________

(a) reverse genetics

(b) protein engineering

(c) mutation

(d) location of function

The question was asked in an interview for internship.

I need to ask this question from Removal and Introduction of Restriction Sites and Generation of Insertions and Deletions topic in section Modifications and Mutagenesis of Genetic Engineering

Answer 1

The correct option is (a) reverse genetics

For explanation I would say: Reverse genetics is the technique by which the exact function of the gene cloned is known. It is done by inactivating the mutant strain in the host.