Question

The principle of gene disruption is ________ to replace the endogenous chromosomal copy of a gene with __________

(a) homologous recombination, inactivated gene

(b) reciprocal translocation, inactivated gene

(c) homologous recombination, activated gene

(d) reciprocal translocation, activated gene

This question was addressed to me in an online interview.

My doubt stems from Choosing the Right Mutations, Database of Mutant Lines and Gene Disruption in section Modifications and Mutagenesis of Genetic Engineering

Answer 1

Right answer is (a) homologous recombination, inactivated gene

The best I can explain: For gene disruption, the basic principle is homologous recombination to replace the endogenous chromosomal copy of a gene with the inactivated gene.