Question

If the target organism contains more than one copy of the gene, what is the effect on these copies?

(a) Only copy is disrupted

(b) All the copies are disrupted

(c) It is difficult to ensure that all the copies are disrupted

(d) Only a specified number of copies are disrupted

This question was posed to me during an interview.

My enquiry is from Choosing the Right Mutations, Database of Mutant Lines and Gene Disruption in section Modifications and Mutagenesis of Genetic Engineering

Answer 1

The correct choice is (c) It is difficult to ensure that all the copies are disrupted

For explanation I would say: If more than one copy of the gene is there in the target organism, it is difficult to ensure that all the copies are disrupted. Multiple copies of the gene can be present, if the organism is not haploid.