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How much effect is there on the surrounding genes by the loxP sequence which is left after recombination has taken place?

(a) Little effect

(b) No effect

(c) Huge effect

(d) It depends on the nature of the surrounding gene

The question was posed to me during a job interview.

I would like to ask this question from Cre Lox Excision in portion Modifications and Mutagenesis of Genetic Engineering

1 Answer

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Correct option is (b) No effect

To elaborate: As the recombination has taken place, the loxP sequence remains there. The remaining loxP sequence has no effect on the surrounding genes.

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