Question

If there are multiple restriction sites for an enzyme in a given molecule, digestion is carried out in many steps. The initial digestion should be?

(a) complete

(b) partial

(c) carried out by a different enzyme then that the restriction site

(d) carried out by a mixture of restriction enzymes

I had been asked this question in an interview.

I need to ask this question from Removal and Introduction of Restriction Sites and Generation of Insertions and Deletions topic in section Modifications and Mutagenesis of Genetic Engineering

Answer 1

Correct option is (b) partial

Easiest explanation: If there are multiple restriction sites for an enzyme in a given molecule, the initial digestion would be partial. It is so because molecules cut on one site at an average.