It is easier to subclone a restriction fragment if it belongs to?

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If mixed oligonucleotides are used, it is regarded as ______________

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How many sites can be mutated at a time?

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Which of the following statement is incorrect for the synthesis of the second strand?

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Once the double stranded molecule with the mutation is introduced into E. coli for replication, how many types of molecules are produced?

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Several different mutations can be induced at one site.

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When a series of deleted regions are replaced by some other DNA fragment of equal length, then it is known as:

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In the case of deletion of a restriction site, it should be cleaved with the same restriction enzyme.

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If a functional gene is disrupted while disrupting a restriction site _______ is created.

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If there are multiple restriction sites for an enzyme in a given molecule, digestion is carried out in many steps. The initial digestion should be?

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Restriction site can also be introduced by oligonucleotide directed mutagenesis of a region that is _______ and ______ to the original restriction site.

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Small deletions at a restriction site can be generated by cutting and degrading the ________ with an exonuclease.

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If PCR is used to introduce random mutations rather than specific mutations, it is called as ___________

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If methods are based on cellular processes that lead to inactivation of gene expression by affecting the RNA, then it is called as ___________

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Additional restriction sites can be introduced near the existing restriction site by mutagenesis.

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PCR using the mutagenic primer and one of the flanking primers is used to carry out amplification and generates a product corresponding to the part of the gene. It is called as megaprimer.

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When inserting a DNA fragment, it is possible to have two orientations. If the orientation is controlled, this cloning is referred to as __________

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It is often useful to inactivate endogenous genes in an organism. It might be helpful in finding out _________ role of the wild type gene.

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At times, the gene which is cloned is not well known for the protein encoded by it. To access the function, the endogenous gene for the mutant strain is inactivated. This technique is called as ___________

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Sometimes mutagenesis is carried out with the help of primers. Choose the correct statement with respect to it.

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Cre can be introduced by crossing it with a strain containing the gene or by infection with virus containing it.

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Stable acquisition of the marker can take place only if a double crossover over the flanking sequence and their chromosomal counterparts causes the marker’s integration into the chromosome.

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