Question

When inserting a DNA fragment, it is possible to have two orientations. If the orientation is controlled, this cloning is referred to as __________

(a) forced cloning

(b) orientation cloning

(c) correct cloning

(d) restricted cloning

I have been asked this question by my college professor while I was bunking the class.

I'd like to ask this question from Modifications in Cloning Methods, Linkers,  Adaptors and Cassettes topic in division Simple Cloning of Genetic Engineering

Answer 1

Correct answer is (a) forced cloning

To explain: It is possible to insert the DNA fragment into two possible orientations. If somehow it is controlled to insert the fragment in a particular orientation, it is known as forced cloning.