Question

Which of the following orientation is not propagated to later stages?

(a) Circular plasmids having genomic DNA

(b) Intramolecular ligation of plasmids

(c) Linear molecules

(d) Head to head ligation of two DNA plasmids

I got this question by my school teacher while I was bunking the class.

The query is from Cloning using a Simple Plasmid and Alpha Complementation topic in portion Simple Cloning of Genetic Engineering

Answer 1

Right choice is (c) Linear molecules

For explanation I would say: When a cloning experiment is carried out, there various orientations which are possible. The orientation which is not propagated to the later stages is a linear molecule. Other possible orientations are relegation of plasmid, intramolecular ligation of genomic DNA. Intermolecular ligation of two DNA plasmids and genomic plasmids is also possible.