Question

Transformation efficiency is defined as __________

(a) ratio of transformed colonies by microgram of sample DNA that is to be inserted

(b) ratio of transformed colonies by amount of sample DNA that is to be inserted

(c) ratio of transformed colonies by microgram of plasmid DNA

(d) ratio of transformed colonies by amount of plasmid DNA

I have been asked this question by my college professor while I was bunking the class.

My enquiry is from Cloning using a Simple Plasmid and Alpha Complementation topic in chapter Simple Cloning of Genetic Engineering

Answer 1

The correct option is (d) ratio of transformed colonies by amount of plasmid DNA

Explanation: Transformation efficiency is defined as the ratio of transformed colonies to that of the amount of plasmid DNA which is taken. It is often altered by altering the competence of the cells.