Question

Insertion of DNA into lacZ gene may lead to disruption of the gene function.

(a) True

(b) False

This question was posed to me in an internship interview.

Question is taken from Cloning using a Simple Plasmid and Alpha Complementation in portion Simple Cloning of Genetic Engineering

Answer 1

Right option is (a) True

To elaborate: Insertion of DNA into lacZ gene may lead to disruption of the gene function. It always doesn’t so but there are minimal chances that function is not disrupted because often it leads to shifting of the reading frame.