Question

Often PCR can be performed in order to confirm whether an insert is present in the plasmid. Cells are taken directly and PCR is performed, this type of PCR is known as __________

(a) direct PCR

(b) colony PCR

(c) quantitative PCR

(d) in-situ PCR

I got this question in my homework.

I need to ask this question from Cloning using a Simple Plasmid and Alpha Complementation in portion Simple Cloning of Genetic Engineering

Answer 1

The correct choice is (b) colony PCR

The explanation: When cells from colonies are taken and directly PCR is performed, it is called as colony PCR. Primers anneal to each side of the cloning site and there is no need of purification in such cases.