Question

Bla is a gene, which is incorrect for it?

(a) It is an antibiotic resistance genes

(b) Antibiotic acts by blocking the cross-linking of the bacterial cell wall and thus leading to lysing of cells

(c) It encodes beta lactamase enzyme

(d) The beta lactam ring is activated

I had been asked this question during an online interview.

Query is from Cloning using a Simple Plasmid and Alpha Complementation in division Simple Cloning of Genetic Engineering

Answer 1

Correct option is (d) The beta lactam ring is activated

Easy explanation: Bla is an ampicillin resistant gene. Antibiotic acts by blocking the cross-linking in the bacterial cell wall and thus leading to lysing of the cells. If antibiotic resistant gene is used, which encodes for beta lactamase enzyme it inactivates the beta lactam gene by hydrolysing it.