Question

TA cloning is a method used for cloning of PCR products. Which of the statement is correct with respect to it?

(a) It is based on the fact that a T residue is incorporated at the end of the PCR product

(b) ‘A’ residue is incorporated into the end of the vector

(c) It gives a low yield

(d) It is preferred over blunt end ligation

The question was posed to me by my school teacher while I was bunking the class.

This intriguing question originated from Modifications in Cloning Methods, Linkers,  Adaptors and Cassettes topic in chapter Simple Cloning of Genetic Engineering

Answer 1

Correct choice is (d) It is preferred over blunt end ligation

Best explanation: TA cloning is a method used for cloning of PCR products. It is based on the fact that A residue is present at the 3’ end of the PCR product and thus now T residue is incorporated at the end of the vector. It is preferred over blunt end ligation.