Question

What is the disadvantage of amplification of using PCR over natural cloning?

(a) In PCR, there is no proof reading activity

(b) In PCR, small fragments can’t be amplified

(c) There is an A incorporated in PCR products, which makes cloning difficult

(d) PCR takes more time as compared to natural cloning

The question was asked during a job interview.

The question is from Modifications in Cloning Methods, Linkers,  Adaptors and Cassettes in chapter Simple Cloning of Genetic Engineering

Answer 1

Correct option is (a) In PCR, there is no proof reading activity

The explanation is: The biggest disadvantage of using PCR amplification over natural cloning is that there is no proof reading activity in the case of PCR. Thus if an error is induced, it is carried forward and is amplified. Also, if large fragments are to be cloned, natural cloning is preferred over PCR. The incorporated A at ends makes the process of cloning of PCR products easier.