Question

Multiple cloning site (MCS) is defined as __________

(a) site within the plasmid which contains a site for many restriction enzymes

(b) site within the plasmid which contains a site for many restriction enzymes and they are not present anywhere else in the plasmid

(c) as the site containing many sites for only one restriction enzyme

(d) cloning many inserts together

I have been asked this question in an internship interview.

I would like to ask this question from Vectors in section Simple Cloning of Genetic Engineering

Answer 1

The correct option is (b) site within the plasmid which contains a site for many restriction enzymes and they are not present anywhere else in the plasmid

Easiest explanation: MCS is defined as a location in the plasmid which is having the site for many restriction enzymes and they are not present anywhere else in the plasmid.