Question

The correct statement with respect to lacIq is __________

(a) It is a mutant of lac repressor gene and decreases the level of repressor

(b) It doesn’t control the lacZ gene

(c) It is important for the vectors to replicate in a low copy number

(d) It doesn’t titrate out the repressor produced by wild type Lac-I gene

The question was asked during an online interview.

My question is based upon Transformation and Hosts in section Simple Cloning of Genetic Engineering

Answer 1

Correct answer is (a) It is a mutant of lac repressor gene and decreases the level of repressor

Easy explanation: It is a mutant of lac repressor gene and decreases the level of repressor. It controls the lacZ gene and it is important for the vectors to replicate with a high copy number. It titrates out the repressor produced by wild-type lac-I gene.