Login

Remember

Register

CarrierAdda QnA

Q&A
Hot!
Unanswered
Tags
Users
Ask a Question

Home
Genetic Engineering
It is also possible to generate a fusion protein...

It is also possible to generate a fusion protein in which ______ is encoded by the vector.

← Prev Question Next Question →

+1 vote

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

It is also possible to generate a fusion protein in which ______ is encoded by the vector.

(a) N terminal

(b) C terminal

(c) Both N and C terminal

(d) Middle region

The question was asked in homework.

Question is from Expression in Vivo topic in section Use of Cloned DNA of Genetic Engineering

genetic engineering
use of cloned dna
expression in vivo

Facebook Twitter LinkedIn

Please log in or register to answer this question.

1 Answer

+1 vote

answered Feb 9, 2022 by Noah (693k points)
selected Sep 18, 2022 by Charlotte

Best answer

The correct answer is (b) C terminal

For explanation: Generally, N terminal is encoded by the vector. But at times it is also possible to generate a fusion protein by encoding C terminal.

Please log in or register to add a comment.

Related questions

+1 vote

1 answer

If an expression vector contains all three, viz. a controllable promoter, a ribosome binding site and an initiation codon, then a hybrid protein is produced. The _______ region of hybrid protein is encoded by vector and the rest is expressed by the sequence inserted.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
use of cloned dna
expression in vivo

+1 vote

1 answer

In fusion proteins, the reading frame in a vector is out of phase with the insert.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
use of cloned dna
expression in vivo

+1 vote

1 answer

Proteins synthesized by carrying out a translation of the vector region and continuing it in the insert region is called as _____________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
use of cloned dna
expression in vivo

+1 vote

1 answer

There are some advantages of expressing protein as a fusion protein. It may enhance stability, folding ______ and ______ formation.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
use of cloned dna
fusion proteins

+1 vote

1 answer

Termination codon is required for stopping high levels of transcription of other regions such as _______ which might interfere with ___________ of the vector.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
use of cloned dna
expression in vivo

+1 vote

1 answer

If overexpression of proteins takes place, they become toxic at times. In such a case _______ colonies are recovered after ______ with the recombinant plasmid.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
use of cloned dna
expression in vivo

+1 vote

1 answer

For co-expression of proteins, it is possible to have several independent sets of promoters and other signals in a single vector.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
use of cloned dna
co-expression of proteins and optimization

+1 vote

1 answer

Expression of cloned sequences in E.coli requires a promoter, a ribosome binding site and an initiation codon.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
use of cloned dna
expression in vivo

+1 vote

1 answer

Which of the following is present in E. coli rrnB operon for rRNA?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
use of cloned dna
expression in vivo

+1 vote

1 answer

Ribosome binding site in E.coli is composed of which sequence primarily?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
use of cloned dna
expression in vivo

+1 vote

1 answer

Translation starts at first _____ codon in the mRNA downstream from the ribosome binding site.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
use of cloned dna
expression in vivo

+1 vote

1 answer

Insertion of vector into intron gives rise to ________ it contains a part coming from intron and a part from ______

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
use of cloned dna
gene function and gene trapping

+1 vote

1 answer

Often, protein to be expressed is fused with histidine and it is called as histidine tags. For their purification, matrix containing ______ is used.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
use of cloned dna
fusion proteins

+1 vote

1 answer

Secretion of overexpressed proteins by fusion to a secretion signal can be useful because it helps in bringing about correct _______ and _____ protein folding.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
using other organisms
other systems

+1 vote

1 answer

At times, the gene which is cloned is not well known for the protein encoded by it. To access the function, the endogenous gene for the mutant strain is inactivated. This technique is called as ___________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
modifications and mutagenesis
removal and introduction of restriction sites and generation of insertions and deletions

+1 vote

1 answer

Maltose binding protein is the product of ______ gene in E.coli and located in ______

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
use of cloned dna
fusion proteins

+1 vote

1 answer

Pel B protein is produced in plants and helps in the degradation of ______

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
use of cloned dna
fusion proteins

+1 vote

1 answer

It is not possible to solubilise proteins from aggregated mass of proteins.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
use of cloned dna
co-expression of proteins and optimization

+1 vote

1 answer

If transcription should not be carried out beyond the insert in the vector, then it should be linearized.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
use of cloned dna
synthesis of rna

+1 vote

1 answer

______ fluorescent protein should be used for determination of _______ of subcellular components.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
use of cloned dna
gene function and gene trapping

+1 vote

1 answer

A considerable amount of modification can take place in the ______ region of RNA in _______ before translation. It affects the yield of proteins.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
use of cloned dna
co-expression of proteins and optimization

+1 vote

1 answer

Some vectors carry a mutant form of promoter known as lacUV5 promoter. It carries ______ in the promoter region and _____ the efficiency.

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
use of cloned dna
promoters

+1 vote

1 answer

RNA can be synthesized by using vector. A vector with _______ is used and further through ________ RNA is isolated.

asked Feb 8, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
use of cloned dna
synthesis of rna

+1 vote

1 answer

A short peptide region fused to a protein of interest is known as ___________

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
use of cloned dna
fusion proteins

+1 vote

1 answer

How many methods are there, which are used for protein synthesis in vitro?

asked Feb 7, 2022 in Genetic Engineering by Charlotte (118k points)

genetic engineering
use of cloned dna
synthesis of proteins and translation in vitro

Facebook Twitter LinkedIn

We welcome you to Carrieradda QnA with open heart. Our small community of enthusiastic learners are very helpful and supportive. Here on this platform you can ask questions and receive answers from other members of the community. We also monitor posted questions and answers periodically to maintain the quality and integrity of the platform. Hope you will join our beautiful community

Categories

All categories
Environmental Biotechnology (884)
Environmental Engineering (1.1k)
Enzyme Technology (752)
Farm Machinery (534)
Fermentation Technology (994)
Finite Element Method (480)
Fluid Mechanics (948)
Fluidization Engineering (267)
Food Engineering (984)
Food Packaging Technology (502)
Food Processing Unit Operations (663)
Foundry and Forging (381)
Genetic Engineering (710)
Geotechnical Engineering I (1.2k)
Geotechnical Engineering II (735)
Hazardous Waste Management (880)
Software Architecture (921)
Mass Transfer (499)
Machine Dynamics (772)
Machine Kinematics (912)
Irrigation Engineering (1.2k)
Linear Integrated Circuits (404)
Machine Design (0)
Machine Drawing (0)

Send feedback
About Us
Copyright
Privacy Policy
Terms of Use
Contact Us

Powered by TalkJarvis

...