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Often, protein to be expressed is fused with histidine and it is called as histidine tags. For their purification, matrix containing ______ is used.

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Often, protein to be expressed is fused with histidine and it is called as histidine tags. For their purification, matrix containing ______ is used.

(a) calcium ions

(b) nickel ions

(c) iron ions

(d) fluorine ions

The question was posed to me by my college director while I was bunking the class.

My question is from Fusion Proteins in chapter Use of Cloned DNA of Genetic Engineering

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The correct option is (b) nickel ions

To elaborate: Histidine tags are used in place of naturally occurring protein at times for fusion proteins. For purification, matrix containing nickel ions is used and is based on affinity purification.

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