Question

If an expression vector contains all three, viz. a controllable promoter, a ribosome binding site and an initiation codon, then a hybrid protein is produced. The _______ region of hybrid protein is encoded by vector and the rest is expressed by the sequence inserted.

(a) N terminal

(b) C terminal

(c) Both N & C terminal

(d) Middle

I got this question by my college director while I was bunking the class.

My question is based upon Expression in Vivo topic in chapter Use of Cloned DNA of Genetic Engineering

Answer 1

The correct option is (a) N terminal

Easy explanation: If all three elements are present, then a hybrid protein is formed. The N terminal region of the hybrid protein is encoded by the vector and the rest is expressed by the sequence inserted.