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In fusion proteins, the reading frame in a vector is out of phase with the insert.

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in Genetic Engineering by (118k points)
In fusion proteins, the reading frame in a vector is out of phase with the insert.

(a) True

(b) False

I got this question in an online interview.

My query is from Expression in Vivo in portion Use of Cloned DNA of Genetic Engineering

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The correct answer is (b) False

To elaborate: Fusion proteins are the proteins produced by carrying the translation in vector region and then continuing it in insert region. Hence, to carry out this, the reading frame should be in phase.

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