Question

If overexpression of proteins takes place, they become toxic at times. In such a case _______ colonies are recovered after ______ with the recombinant plasmid.

(a) few, translation

(b) few, transformation

(c) no, transformation

(d) no, translation

I had been asked this question during an online interview.

The doubt is from Expression in Vivo topic in portion Use of Cloned DNA of Genetic Engineering

Answer 1

Correct answer is (c) no, transformation

To elaborate: At times, overexpression of proteins takes place. It leads to toxicity. In such a case, no colonies are recovered after transformation with the recombinant plasmid. It is so because no colonies survive because of overexpression of the protein.