Question

Choose the correct statement for adaptors.

(a) They are blunt ended at both the ends

(b) They are single stranded at both the ends

(c) They may be single stranded at one end and other end may be blunt

(d) They don’t have extra restriction sites within their sequence

The question was asked in an interview for job.

The query is from Modifications in Cloning Methods, Linkers,  Adaptors and Cassettes in portion Simple Cloning of Genetic Engineering

Answer 1

The correct answer is (c) They may be single stranded at one end and other end may be blunt

Easiest explanation: Adaptors are molecules which are used for cloning. There is no restriction on the type of end they have. Either of the ends can be blunt or staggered. They may also have extra restriction site within the sequence.