Question

M13 vector is tightly packed. Where is the available site to insert DNA?

(a) In the gene II

(b) Between gene II and IV

(c) Between gene III and V

(d) Between gene V and VIII

I have been asked this question in my homework.

The doubt is from BAC Vector, M13 and its Derivatives topic in division Other Vector Systems for E of Genetic Engineering

Answer 1

The correct choice is (b) Between gene II and IV

The explanation is: The production of phage depends upon the insertion of DNA without disrupting any of the phage genes. Hence, M13 vector is tightly packed. One such site for the insertion of DNA is between gene II and gene IV. Here, the lacZ and MCS are also present.