Question

Choose the correct statement for BAC vector system.

(a) BAC vector system stands for bacteria and chromosome

(b) It usually accepts insert of size approximately 1000kbp

(c) The repE and oriS sequences are required for controlling the copy number and par A-C sequences are required for replication

(d) A selectable marker is there for chloramphenicol resistance

I got this question during an interview.

Asked question is from BAC Vector, M13 and its Derivatives in portion Other Vector Systems for E of Genetic Engineering

Answer 1

Correct option is (d) A selectable marker is there for chloramphenicol resistance

The best I can explain: BAC vector system stands for Bacterial Artificial Chromosome. It accepts an insert of size 100kbp-300kbp. The repE and oriS sequences are required for controlling the replication and the par A-C sequences are required for controlling the copy number. A selectable marker is there for chloramphenicol resistance. A lacZ gene is also present and recombination of BAC vectors is done into E. coli by electropoartion.