Question

Bacteriophage Mu is also a phage, choose the correct statement for it.

(a) It is an example of temperate phage

(b) It is packaged into heads containing 50 kbp of DNA

(c) When the phage replicates it goes transposition only a few times

(d) Mu can’t be used for cloning in vivo

This question was addressed to me in an interview for internship.

I'd like to ask this question from Bacteriophage-Mu and Bacteriophage-Pi in section Other Vector Systems for E of Genetic Engineering

Answer 1

Correct choice is (a) It is an example of temperate phage

Explanation: Bacteriophage Mu is also a phage, it is an example of temperate phage. It is packaged into heads containing 39 kbp of DNA. When the phage replicates it goes transposition a few hundred times and inserts itself into many places in the host genome. Mu can be used for cloning in vivo as a ‘mini Mu’ that contains reduced phage genome.