Question

Excision of the F factor can also be done from the host chromosome. Choose the incorrect statement in respect to it.

(a) This excision is possible in vivo

(b) Along with the F factor, host chromosome’s DNA sequences are also excited

(c) The sequences of host chromosome which can be accommodated in the F plasmid are small in size

(d) These plasmids are termed as F’ plasmids

I had been asked this question in unit test.

The question is from BAC Vector, M13 and its Derivatives in section Other Vector Systems for E of Genetic Engineering

Answer 1

Right choice is (c) The sequences of host chromosome which can be accommodated in the F plasmid are small in size

To elaborate: It is possible to carry out the excision of the F factor from the host chromosome. The excision is possible in vivo. Along with the F factor, the DNA sequences of host chromosome are also excised. The F plasmid is able to accommodate very large size f host chromosome fragments and these are known as F’ plasmids.