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Packaging in vitro is basically carried out by two different strategies. Choose the incorrect statement for it.

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Packaging in vitro is basically carried out by two different strategies. Choose the incorrect statement for it.

(a) Separate strains with chain-termination mutations in different genes for coat components are used __________

(b) Temperature sensitive cI repressor is used

(c) Due to heat shock packaging proteins are induced

(d) Packaging is carried out in each strain

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My question is from Cloning in Lambda topic in portion Other Vector Systems for E of Genetic Engineering

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The correct choice is (d) Packaging is carried out in each strain

To explain: Packaging in vitro is basically out by two different strategies. In the first strategy, separate strains with chain-termination mutations in different genes (mostly D and E genes) for coat components are used. Temperature sensitive cI repressor is used and due to induction by heat shock, packaging proteins are produced. But packaging is not carried out in any of the strain because none of the strain is having full complement of proteins to carry out packaging.

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