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Is it necessary to stop the relegation of the central portion before the stiffer fragment is ligated.

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+1 vote
in Genetic Engineering by (118k points)
Is it necessary to stop the relegation of the central portion before the stiffer fragment is ligated.

(a) True

(b) False

I got this question in homework.

The question is from Cloning in Lambda topic in section Other Vector Systems for E of Genetic Engineering

1 Answer

+1 vote
by (693k points)
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Best answer
The correct option is (a) True

Best explanation: The central portion should be stopped from religating before the stuffer fragment should be ligated. There are chances that central portion gets religated to the arms.

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