Question

The fragment inserted in the place of the central portion of the genome is known as __________

(a) insertion fragment

(b) substitution fragment

(c) stuffer fragment

(d) displacement fragment

I have been asked this question at a job interview.

My question comes from Cloning in Lambda topic in chapter Other Vector Systems for E of Genetic Engineering

Answer 1

Right answer is (c) stuffer fragment

For explanation I would say: The fragment inserted in the place of the central portion of the genome is known as the stuffer fragment. It is generated by doing partial digestion an enzyme having a 4bp long recognition site.