Question

Packaging in vitro is also carried out to introduce the lambda DNA into the cells. Choose the incorrect statement in respect to it.

(a) Packaging in phage coat is a more efficient process than the normal transformation process

(b) The lambda DNA is incubated with lysate of lambda infected cells in a concatemeric configuration

(c) Lysate constitutes of lambda proteins needed for phage assembly

(d) The amount of non-recombinant phage in the background is less

The question was asked during an online exam.

The query is from Cloning in Lambda topic in division Other Vector Systems for E of Genetic Engineering

Answer 1

The correct choice is (d) The amount of non-recombinant phage in the background is less

The best I can explain: Packaging in vitro is used to introduce the lambda. It is more efficient than the normal transformation procedure. Thus, the lambda DNA is incubated with lysate of lambda infected cells in a concatemeric configuration. This lysate constitutes of lambda proteins needed for phage assembly and hence the recombinant DNA is also packed. But there is also a large amount of non-recombinant packaging in the background.