Question

Choose the correct statement for the lambda ZAP vector.

(a) It is not based on bacteriophage lambda

(b) It contains a region that can be excised in vivo

(c) The excision leads to the formation of bluescript plasmids and it contains an initiator region only

(d) The multiple cloning site is not flanked by the initiator and the terminator region

I have been asked this question in class test.

Question is from Lambda ZAP and Cosmids in portion Other Vector Systems for E of Genetic Engineering

Answer 1

The correct answer is (b) It contains a region that can be excised in vivo

The explanation is: Lambda ZAP vector is based on the bacteriophage lambda. It contains a region that can be excised in vivo and leads to the formation of bluescript plasmids. This consists of multiple cloning site which is flanked by the initiator and the terminator region.