Question

Choose the correct statement for lambda gt10.

(a) It is having an EcoRI site

(b) It is having cII gene

(c) It accepts an insert of size greater than that of 8 kbp

(d) This size is less than that the size which is generally accepted by wild type phage

The question was posed to me in a job interview.

The doubt is from Cloning in Lambda in chapter Other Vector Systems for E of Genetic Engineering

Answer 1

Right answer is (a) It is having an EcoRI site

To elaborate: Lambda gt10 is having an EcoRI site which contains the cI repressor gene. It accepts an insert of size upto 7.6 kbp which is generally more than that of the size generally accepted by a wild type phage.