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Choose the correct statement for RecBCD nuclease.

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in Genetic Engineering by (118k points)
Choose the correct statement for RecBCD nuclease.

(a) It promotes rolling circle replication

(b) It is blocked by Gam protein

(c) It blocks theta mode of replication

(d) It is blocked by Red protein

I got this question in an interview for internship.

The origin of the question is Cloning in Lambda topic in section Other Vector Systems for E of Genetic Engineering

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Right option is (b) It is blocked by Gam protein

The best explanation: RecBCD nuclease is blocked by Gam protein and it blocks rolling circle replication. As the rolling circle mode is blocked, replication would be carried out by only theta mode of replication.

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