Question

Choose the correct statement for Sanger’s method of sequencing.

(a) They are also called as chain termination methods

(b) Use of M13 vector is made for getting double stranded DNA

(c) Use of dideoxynucleotide phosphate (ddNTP) is made

(d) The use of M13 vectors is more preferred these days

The question was posed to me in exam.

Origin of the question is BAC Vector, M13 and its Derivatives topic in portion Other Vector Systems for E of Genetic Engineering

Answer 1

The correct option is (a) They are also called as chain termination methods

The explanation: Sanger’s method of DNA sequencing is also known as the chain termination method of sequencing. Dideoxynucleoside triphospahte (ddNTP) are used as chain terminating agents. M13 vector is used to obtain the single stranded DNA. Now a days there are technologies available for sequencing on double stranded DNA, thus it reduces the use of M13 vectors these days.