Question

M13-plasmid vectors are often used. Which of the following is not a feature for this?

(a) They are known as phagemid, plage or phasmid

(b) Replication takes place through the origin of replication of M13 derivatives only

(c) Replication from M13 requires proteins to be provided by helper phage

(d) Some of the examples of these types of the combination are pUC 118, 119 and 120

This question was addressed to me in an online interview.

I need to ask this question from BAC Vector, M13 and its Derivatives in division Other Vector Systems for E of Genetic Engineering

Answer 1

Right answer is (b) Replication takes place through the origin of replication of M13 derivatives only

Easy explanation: M13-plasmid vector hybridization is often used and they are referred to as phagemid, phasmid or plage. Replication can take place either by the origin of replication of the plasmid or by the origin of replication of M13. Replication from M13 requires additional protein to be provided from helper phage. Single-stranded DNA is produced after replication and is packaged in a protein coat. Some of the examples of this type of hybridization are pUC 118, 119 and 120.