Question

Promoter-probe vectors are used often. Choose the correct statement for these vectors.

(a) They are used for identifying sequences that can function as promoter in vivo

(b) It contains a reporter gene which contains its promoter along

(c) The reporter gene is having a cloning site

(d) After insertion of DNA into the cloning site, selection of plasmids is carried out by blue white screening

I have been asked this question in an interview for job.

This intriguing question comes from Reporter Genes in section Screening Libraries of Genetic Engineering

Answer 1

Right answer is (a) They are used for identifying sequences that can function as promoter in vivo

The best explanation: Promoter- probe vectors are used for identification of sequences that can function as a promoter in vivo. It consists of a reporter gene which is not having its promoter along but other genes such as those for chloramphenicol resistance are present. The reporter gene is preceded by the cloning site and after the insertion of DNA has been done, selection of plasmids is carried out by using chloramphenicol resistance.