Question

Xenopus oocyte cells are also used at the time to carry out screening. Choose the incorrect statement for this procedure.

(a) DNA from collections is transcribed within in vitro and the transcription products are microinjected into oocytes

(b) The RNA is translated within oocytes

(c) Screening is carried out on the basis of DNA

(d) The screening is helpful for the proteins whose function can be easily screened such as transport of ions

The question was asked during an internship interview.

My doubt is from Screening by Expression In Vivo topic in division Screening Libraries of Genetic Engineering

Answer 1

The correct option is (c) Screening is carried out on the basis of DNA

The best I can explain: DNA from collections is transcribed within in vitro and the transcription products are microinjected into the oocytes. The RNA is translated within the oocytes and screening is carried out on the basis of RNA. The screening is helpful for those proteins whose functions can be easily screened such as transport of ions.