Question

Choose the incorrect statement for the open reading frame.

(a) It is helpful in deciding whether the clone is correct or not

(b) It consists of an initiation codon

(c) It consists of a termination codon

(d) The presence of introns doesn’t affect the open reading frame

The question was asked in an interview.

Question is taken from Expression of Coding Section in-Vitro in section Screening Libraries of Genetic Engineering

Answer 1

The correct choice is (d) The presence of introns doesn’t affect the open reading frame

To explain I would say: Open reading frame is very important in deciding whether the clone is correct or not. It consists of an initiation codon, followed by a stretch of DNA and termination codon. The presence of introns the open reading would be affected and makes detection difficult.