Question

Choose the incorrect statement for colony or plaque lift.

(a) It is the base for screening based on nucleic acid hybridization

(b) It is also known as Grunstein-Hogness technique

(c) It is based on the fact that which members of the library have the same sequence as the DNA probe

(d) The library may be plaques on a bacterial lawn and in that case it is known as plaque lift

I had been asked this question in an online quiz.

My question is taken from Database Screening and Screening by Nucleic Acid Hybridisation in portion Screening Libraries of Genetic Engineering

Answer 1

Correct option is (c) It is based on the fact that which members of the library have the same sequence as the DNA probe

The explanation is: Colony lift or plaque lift is the basis for nucleic acid hybridization. It is also known as Grunstein-Hogness technique named after the discoverer. It is based on the fact that which members of the library are complementary to the sequence of DNA probe can be known. The library may be plaques on a bacterial lawn and in that case it is known as plaque lift. There may be colonies on a plate and in that case, it is known as colony lift.