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After hybridization how the position of the labelled DNA probe is determined?

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in Genetic Engineering by (118k points)
After hybridization how the position of the labelled DNA probe is determined?

(a) By staining

(b) By UV irradiation

(c) By using chemiluminescence

(d) By electronic microscope

The question was asked in exam.

Question is taken from Database Screening and Screening by Nucleic Acid Hybridisation in portion Screening Libraries of Genetic Engineering

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The correct option is (c) By using chemiluminescence

The explanation: After the hybridization has been carried out and the membrane has been washed, the position of the labelled DNA probe is determined by using chemiluminescence. Commonly the probe is labelled by incorporation of fluoroscein conjugated molecules.

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