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Which library should be used, genomic or cDNA when the screening is carried out by oligonucleotide probes?

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in Genetic Engineering by (118k points)
Which library should be used, genomic or cDNA when the screening is carried out by oligonucleotide probes?

(a) Genomic

(b) cDNA

(c) Both genomic and cDNA library can be used equivalently

(d) None of the libraries are preferred if oligonucleotide probes are used

I got this question in exam.

This intriguing question originated from Database Screening and Screening by Nucleic Acid Hybridisation topic in portion Screening Libraries of Genetic Engineering

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Correct choice is (b) cDNA

To explain I would say: If oligonucleotide probes are used, then cDNA libraries should be used. It is so because cDNA libraries are enriched for coding sequences and thus fewer sequences have to be screened.

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